Cryo Transmission Electron Microscopy

Cryo-TEM enables the investigation of aqueous samples (liposomes, nanoemulsions, …) where the cellular structure and composition are maintained under physiological conditions. This technique involves rapid freezing of a droplet of an aqueous sample in a cryogen (liquid ethane), which preserves the sample in a vitreous ice layer on a standard Cu grid supported with a holey carbon film. The sample is kept frozen at liquid nitrogen temperature during the investigation in the TEM.

 

heller_liposomes_41x BF

Liposomes